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Cells were cultured in 24 well plates and treated with GHK or GHK-PEG at 1 or 10 nM for 24 h

There remains contention over whether hyperacetylation is driven by upregulation of acetylating enzymes, or spontaneous acetylation reactions due to high concentration of acetyl-CoA in mitochondria

Mechanistically, Salidroside enhances the phosphorylation of AMPK, PI3K/Akt, and GSK3 in HepG2 cells (Zheng et al., 2015), thereby inhibiting hepatic gluconeogenesis through reduced AMPK-mediated expression of PEPCK and G6Pase

We first applied an acute dose of 0.1 mM H 2 O 2

7 Structural basis of Pt GSTF8 dehydratase activity

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