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Although, usually, several months of high caloric diet are necessary to develop these morbid conditions, short-term exposition to this type of diet is sufficient to induce metabolic changes that can favor liver injury susceptibility

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10.1016/j.bcp.2013.06.002 Biochem

S-5-HT was measured with the HPLC-ECD chromatographic method (38) using a fully clotted sample to accurately take into account the serotonin in circulating platelets

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Two molecules of acetyl-CoA condense to form HMG-CoA, which is then reduced to mevalonate (MVA) by the rate-limiting enzyme HMGCR with NADPH consumption, and subsequently converted to cholesterol in the endoplasmic reticulum via squalene and lanosterolan energy-intensive process requiring ATP and NADPH ( Figure 1 )