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mRNA quantification by quantitative real-time PCR (qRT-PCR) Total RNA was isolated using QIAzol-Reagent (Qiagen, Germany), based on modified Chomczyski & Sacchis method (Chomczynski and Sacchi, 2006), following the manufacturers protocol

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K.DaltonP.DingX.FinelM.2009Human UDP-glucuronosyltransferase UGT2A2: cDNA construction, expression, and functional characterization in comparison with UGT2A1 and UGT2A3.1744-687217446872 102