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The review concluded that NNMT plays important roles in obesity and type 2 diabetes and validated 5-Amino-1MQ as a potent small-molecule NNMT inhibitor that reverses diet-induced metabolic changes, establishing it as a primary research tool for the NNMT pathway

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5A-1MQ-treated mice switched from WD to LD (WD/LD-T) showed a rather reduced relative abundance of Akkermansia compared to vehicle-treated DIO animals switched from WD to LD (WD/LD-V), suggesting a unique microbiome mechanism associated with weight loss in the vehicle-treated group switched from WD to LD compared to the 5A-1MQ-treated group that underwent the same diet switch