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Description

This imbalance is what we call oxidative stress, and its a root cause of the ageing process and many age-related illnesses

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Briefly, 30 g protein extract of each sample was reduced with 20 mM dithiothreitol for 45 min at 56C, alkylated with 50 mM iodoacetamide for 45 min in the dark, and then digested with trypsin overnight