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Retrieved from Hunt, A., Harrington, D., & Robinson, S

For immunofluorescence, cryosections were dried for 2 h at RT, fixed in pure acetone for 10 min and blocked with PBS 10% FCS for 30 min at RT

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Rudin CM, Yang Z, Schumaker LM, VanderWeele DJ, Newkirk K, Egorin MJ et al

We identified AEP trails as having distinct morphology (distinguishable from membrane vesicles and tubule-like outer membrane protrusions, as well as the S-motility-related EPS fibrils) in the TEM, and by their pH sensitivity, as treatment with acidic stains (un-buffered uranyl acetate (UA), pH 4.5 or SiPTA at pH 4.0) removed slime trails (but not other membrane components, i.e., vesicles) from grids, while neutral stains (SiPTA at pH 7.0) did not (Fig

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