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First, SELECT was not a primary prevention trial, and the data should not be extrapolated to all individuals with overweight and obesity to prevent major adverse CV events

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Statistical analysis of data All experiments were conducted in triplicate

THP1 cells were cultured in RPMI medium supplemented with phenol red (#61870036 ThermoFischerScientific, Darmstadt Germany) and 10 % FCS (fetal calf serum, Brazil) #10270106, ThermoFischerScientific, Darmstadt Germany) at 37 C, 5 % CO 2 and 95% relative humidity

Conclusion: These data suggest that claudin-5 plays an intricate role in the regulation of the BBB and disruption in the potential splicing of this protein may lead to worsened stroke outcomes