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However, it remains critical to mention that glycine transporter inhibitors may be associated with adverse effects and complications, such as gastrointestinal disturbances and systemic toxicity

Impact of high-throughput screening in biomedical research

The significantly higher numbers of peripheral RGCs in the KD + OHT group versus Control are consistent with our previous study that showed the neuroprotective effect of the ketogenic diet in the DBA/2 J model of glaucoma (Harun-Or-Rashid et al., 2018)

Studies in SLC25A19 knockout mice and in carriers of the SLC25A19 mutation, responsible for the Amish lethal microcephaly (MCPHA, see below) show, respectively, virtually undetectable and markedly reduced mitochondrial TPP levels in mouse embryonic fibroblasts and human lymphoblasts (56), which were completely restored upon addition of TPP to the PDH and KGDH complex assay mixtures (56)

Additionally, the authors would like to thank the Dean of the College of Science at University College (Iraq) for their support and encouragement throughout the completion of the protocol

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