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.2020.11.011 PubMed PMID: 33242662

To assess whether Cas12f delivery via EVs requires MIM, we attenuated protrusion formation by introducing a five-lysine-to-alanine (5KA) mutation in the MIM I-BAR domain 54 , which was previously shown to impair EV generation 40

2.14 In vitro angiogenesis (tube formation) assay The in vitro angiogenic activity of HUVECs (human umbilical vein endothelial cells) was determined by the Matrigel tube formation assay

Chromatin as a key consumer in the metabolite economy

Samples were incubated for 1 h and centrifuged at 14,000 g at 4 C for 20 min