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In future studies, analysis via western blot and RT-qPCR

Oral epithelial dysplasia and premalignancy

Upon binding to cytoplasmic glucocorticoid receptors (GR), corticosteroids induce translocation of GR complexes into the nucleus, where they interact with glucocorticoid response elements (GREs) to upregulate anti-inflammatory genes (e.g., IL-10, DUSP-1) and suppress pro-inflammatory mediators like IL-1, IL-6, TNF-, and chemokines, such as MCP-1 and IL-8 (McManus 2003

By maintaining excess GR, hydroperoxide, GSH, and NADPH, the reaction rate becomes solely dependent on GPx activity, enabling continuous, real-time kinetic measurement (Figure 2C)