5-(and-6)-carboxyl-2',7'-dichlorofluorescein diacetate linker 587 SMILES: CN(C)C1=CC=C2C(OC3=CC(C=CC3=C2C2=CC=C(C=C2C([O-])=O)C(=O)NCCN2C(=O)C=CC2=O)=[N+](C)C)=C1 Purity (HPLC):
5-(and-6)-carboxyl-2',7'-dichlorofluorescein diacetate linker 587 SMILES: CN(C)C1=CC=C2C(OC3=CC(C=CC3=C2C2=CC=C(C=C2C([O-])=O)C(=O)NCCN2C(=O)C=CC2=O)=[N+](C)C)=C1 Purity (HPLC):5 (and 6) carboxyl 2,7 dichlorofluorescein diacetate (Carboxy DCFDA, CDCF) can passively diffuse into cells and is colorless and nonfluorescent until the acetate groups are cleaved by intracellular esterases to yield the fluorescent fluorophore, 5 (and 6) carboxy 2,7 dichlorofluorescein. With a pKof 4. 8, this dye can be used as an acidic pH sensor. It has been used as a fluorescent substrate probe to study the distribution and mechanism of action of
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