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Note C-terminal 6xHIS tag (underlined) is included in the pET21 bacterial expression vector

[DOI] [PMC free article] [PubMed] [Google Scholar] 132.Frank DN, St Amand AL, Feldman RA, Boedeker EC, Harpaz N, Pace NR

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1), fluorescence was measured by flow cytometry (ex 488 nm/em 520 nm) (FACSCanto II, BD)