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HbX lowers blood pressure in mice with angiotensin II-induced hypertension and blunts vasoconstrictive response to phenylephrine in isolated human vessels (Keller et al., 2016)

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The eluted enzyme was further subject to dialysis for 12 h [Tris-HCl (100 mM, pH 7.4), DTT (1 mM), and glycerol (20% v/v)] to remove the bulk of imidazole

Further, the pH tended to increase with increasing l-carnitine content and varied in the range of 3.65.5 (Fig