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This approach has allowed for the identification of potential QTLs and causal genes through high-resolution mapping facilitated by linkage disequilibrium (LD) analysis (Liu et al., 2018)

For example, enhancing oxidative phosphorylation in microglia might promote a more quiescent, neuroprotective state, while targeted inhibition of glycolysis could reduce harmful inflammation (Barros et al., 2009

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Optional: If the lysate is too viscous, add RNase A (10 g/mL) and DNase I (5 g/mL) and incubate on ice for 10-15 minutes