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Glutathione disulfide sensitizes hepatocytes to TNF-mediated cytotoxicity via IKK- S-glutathionylation: a potential mechanism underlying non-alcoholic fatty liver disease

Psychiatry 82 KasaharaT.KubotaM.MiyauchiT.NodaY.MouriA.NabeshimaT.et al (2006)

S-Cys-G-S-Cys-Cys-K...-COOH) [22]

The GSH levels of the BSO-treated cells quantified using our RT-based flow cytometry method is well correlated to the values measured using cell lysates with a glutathione reductase-based biochemical assay ( R 2 =0.99, Fig

O 2 , subsequently, is rapidly converted into H 2 O 2 by SOD3, which is tethered to the cell surface

However, these cells are highly sensitive to their environment, and elevated oxidative stress can physically trigger them to degranulate inappropriately