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After an additional 4 days ( t = 96 h), cells were collected by trypsinization, pelleted, snap-frozen and stored at 80 C

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In the case of adhI , its promoter was shown to be activated by both formaldehyde and methanol, although induction by formaldehyde occurs more rapidly and at a lower concentration ( adhI is controlled by two separate, two-component regulatory systems: (i) AfdRS, which activates transcription in the presence of formaldehyde, and (ii) RfdRS, which is thought to act as a repressor, although the signal for derepression is unknown ( afdR and afdS are organized within an operon, as are rfdR and rfdS

The analysis was performed separately for upregulated and downregulated proteins with a fold change greater than 1.5 against biological processes of Gene Ontology (Ashburner et al