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After removal of the supernatant, the aggregates were resuspended in 3.5 ml/million cells of differentiation medium comprised of RPMI 1640 (Life Technologies) with B-27 either with or without insulin (RPMI/B-27 +/-) (Life Technologies) depending on the cell line and 3 or 4 M CHIR99021 (BOC Sciences)

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Toxicology 265 , 115121 (2009)

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Importantly, patients reported that the educational elements were both comprehensible and valuable in supporting their opioid reduction process

The greatest protection was found at 10 6 M (1 M), where only a minor decrease from control hair cell survival scores was observed