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To eliminate the developmental effects of Pc , we did experiments with UAS-Dicer to enhance the RNAi effects and Gal80 ts to temporally induce Pc RNAi in the adult stage

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showed that TMAO production was dependent on the gut microbiota and that mice treated with antibiotics failed to provide the required gut microbiota for TMAO production ( ApoE / mice without changing circulating cholesterol levels (75)

To enhance sensitivity, specificity, and translational relevance, future research should incorporate multimodal biomarker strategiescombining imaging, fluid-based, and multi-omics analyses