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Providing the cells with a PARP inhibitor or an NAD + precursor partially rescued their viability

These methodologies are demonstrated on an antibody fragment, and serve to expand the scope of disulfide bridging strategies whilst offering a convenient route to the construction of multifunctional antibody fragment conjugates

The samples were subsequently washed with 1 ml of 1% BSA and 0.5% Tween-20 in PBS, centrifuged and the pellets were resuspended in 1 ml of PI solution (20 g ml 1 propidium iodide (Sigma-Aldrich, P4170), 0.5 mg ml 1 RNase A (Sigma-Aldrich, R5503) and 0.1% Triton X-100 in PBS)

Hang B, Jassem E, Mohammed H, Wan LQ, Herschkowitz JI, Fan J

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Biochim Biophys Acta - Mol Cell Biol Lipids 1864:213223