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National survey of Japanese patients with mevalonate kinase deficiency reveals distinctive genetic and clinical characteristics

2021b) (Yang et al

Tapering off trazodone under medical supervision is recommended to minimize withdrawal effects

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For cloning, the amplified products were purified using QIAquick PCR Purification kit (Qiagen Inc) and incubated at 72 C for 10 min in a reaction mixture (25 L) containing 200 M of dATP, 1.5 mM MgCl 2 , 0.625 unit of Taq DNA polymerase and 1X buffer, to add A-tail