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(3) Equilibrate the column with 3 bed volumes of ultrapure water (4) Close the bottom outlet of the column 3

Electron microscopy Cells were washed with PBS and centrifuged at 2000 rpm for 5 min

10.1016/j.tiv.2010.04.007 131 MetushiI

Being polyphenols emitting at 280 and 320 nm extracted by PBS together with total soluble proteins (anthocyanins are not efficiently extracted, Supplementary Figure 4), the contribution of these classes of molecules may be hypothesized in stabilizing proteins, as proofs of the interaction of anthocyanins with proteins seem to suggest (Sui et al., 2018)

4B) at 3 hpi, which remained at basal levels

2019 May;25(4):1175-1184