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Chiral HPLC or Marfey analysis may be warranted to confirm correct D-Trp and D-Phe stereochemistry, as racemization during synthesis can produce less active or inactive diastereomers

Since GSH interacts with the 1st glutathione-interaction site, glutathionylated products of the random bi-uni mechanism might already adopt a suitable orientation as classical GSSR substrates for the subsequent deglutathionylation (Fig

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