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Silencing of RRM1 in HT-1080 Control cells decreased the corresponding protein levels and led to compensatory up-regulation of RRM2, indicating that our silencing reagent worked as expected (Fig 5A)

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We next optimized a NRF2 transcriptional signature resulting in a set of 59 genes based on their contribution to the model and overlap with previously reported NRF2 signatures (Figs