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The 20 most intense multiply charged ions (z 2) were sequentially isolated and fragmented in the octopole collision cell by higher-energy collisional dissociation (HCD) using normalized collision energy of 32 with an AGC target of 1 10 5 and 200 normalized AGC targets (%) at 45,000 resolutions

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The consequence is easy to understate: a substantial fraction of the evidence that DSIP exists endogenously and varies with sleep rests on assays that cannot fully distinguish DSIP from related material