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J Ethnopharmacol 155(1):80103 Verma SK, Kumar A (2011) Therapeutic uses of Withania somnifera (Ashwagandha) with a note on withanolides and its pharmacological actions

The elution profile of the sample homogenized in BSAN buffer (blue tracing) is characterized by the presence of both the two symmetrical peaks corresponding to the GS-NEM conjugate (4.29 min and 4.95 min r.t.) and the peak corresponding to the excess of NEM (5.8 min r.t.)