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Together with previous work showing that perturbations of the N-terminal loop or -SNAP/Sec17 membrane engagement compromise SNARE complex disassembly and membrane fusion dynamics 11,37,38 , these findings support the idea that the M105I mutation may impair membrane fusion not only by diminishing plasma membrane binding, but also by diverting -SNAP into non-productive, off-pathway membrane contacts that cannot support a properly assembled 20S complex

Future studies should investigate the factors of where patients live, eat, and work that drive poor medication adherence

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10.1038/s41380-021-01073-6 137

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The activity of peroxidase (POD) was determined by a specialist kit (BC0090) provided by Beijing Solebao Science Technology Co., Ltd