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Because recently developed hybrid compounds have side effects comparable to commercial AChE inhibitors, research has focused on developing selective, potent, and reversible AChE inhibitors

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[DOI] [PMC free article] [PubMed] [Google Scholar] 100.Soliman S., Ahmed R.M., Ahmed M.M., Attia A., Soliman A.R

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The plate was then incubated at 37 C for 45 minutes in a shaker water bath then cooled and the absorbance of the pink colored chromophore was measured at 540 nm using ELISA reader, against a blank treated in a similar manner to the test but using 100l distilled water instead of the sample