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Together, these findings position cigarette butts as critical nodes in the urban resistome, emphasizing the need for targeted management and One Healthoriented interventions. The World Health Organization describes the One Health framework as an integrated, unifying approach that aims to sustainably balance and optimize the health of people, animals, and ecosystems.

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BMJ 330 , 223 (2005)

Comparisons among more than two groups were performed using one-way analysis of variance (ANOVA) followed by Tukeys test or by two-way ANOVA followed by the Bonferroni post hoc test