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Briefly, sgRNA-expressing mCherry + cells were sorted as follows: GFP screen (brightest 30% GFP + and dimmest 30% GFP + ), BFP screens (brightest 30% BFP + and dimmest 30% BFP + ) and GFP:BFP screens (brightest 30% GFP:BFP + ratio and dimmest 30% GFP:BFP + ratio)

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