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Similarly, Rajegowda et al

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Assuming you followed the above advice and used an appropriate binding buffer and your protein isnt huge, then a likely reason for this is that the expression yield is much lower than you expected

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Delphinidin has been demonstrated to inhibit the PI3K/Akt/mTOR signaling pathway in imiquimod-induced psoriasis, which is frequently upregulated in psoriatic lesions and contributes to the excessive proliferation of keratinocytes (Chamcheu et al