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RNCs were eluted by TEV cleavage - revealing the NMT1 target sequence - in APS Buffer supplemented with 0.5 mg/mL TEV protease (homemade) for 1.5 h at 4 C and collected by centrifugation

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Stampfer MJ, Malinow MR, Willett WC, Newcomer LM, Upson B, Ullmann D, Tishler PV, and Hennekens CH (1992)

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