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3, set up calibration wells, sample diluent wells, blank wells and sample wells, and add different concentrations of calibration wells to each calibration well50L, add sample diluent to the sample diluent well50L, no addition is made to the blank wells, and the sample wells are added with the sample to be tested50LBiotin antibody was added to each well except the blank well.100uL, cover the reaction plate with a sealing film,37Incubate in a water bath or incubator away from light60min

To sum up, one of the key pathogenic factors of lethal HF is the irreversible dysfunction and death of terminally differentiated cardiomyocytes (Fang et al., 2019)

Exploring whether hyper-inflammation might be impacted by the presence of TM on monocyte surfaces could provide insights into its potential regulatory role

Oxford University Press, New York Hammer , Harper DAT, Ryan PD (2001) PAST: paleontological statistics software package for education and data analysis

Cellular mechanisms underlying eosinophilic and neutrophilic airway inflammation in asthma

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