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Briefly, the skeletal muscles were homogenized in ice cold buffer (0.25M sucrose, 20mM Tris, 20mM boric acid, 1mM EDTA, 1mM l-serine, and pH 7.4), and a supernatant was obtained by centrifuging at 20,000 g for 30min at 4C

Following progression to first-line chemotherapy, the randomized phase III ABC-06 clinical trial established FOLFOX as the chemotherapy of choice 312

It is important to note that these strategies are not FDA approved to prevent, mitigate, treat or cure kidney stones and should not be confused as such