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additionally, live-cell imaging was performed and the results analyzed according to a protocol described previously (Doanlar et al., 2020[15], 2021[16]) using the NucBlue Live Ready Probes Reagent, CM-H 2 DCFDA dye (Life Technologies, USA), and acridine orange-ethidium bromide (AO/EB) (Sigma Aldrich, USA)

Zhu H, Huang J, Chen Y, Li X, Wen J, Tian M, Ren J, Zhou L, Yang Q (2022) Resveratrol pretreatment protects neurons from oxygen-glucose deprivation/reoxygenation and ischemic injury through inhibiting ferroptosis

Measurements of the rats motor activity included the number of crossed lines (horizontal activity), the frequency of rearing (vertical activity), and the amount of time the rat spent in the center (latency time) [49]

HBV DNA levels impact the prognosis of hepatocellular carcinoma patients with microvascular invasion

Speech-language Pathology Speech-language pathology involves treating communication disorders

Example: 180lb person (82kg) for injury recovery at 5 mcg/kg: 82kg 5mcg = 410mcg per day With 5mg vial + 2ml water = 2,500mcg/ml concentration Draw 0.16ml per injection 12 doses per vial HOW For injury recovery (sprains, tears, tendonitis): Dose: 400-600mcg per day Frequency: Split into 2 doses (morning and evening) Duration: 4-6 weeks minimum Injection site: Near injury OR systemic (abdomen) Results timeline: 2-3 weeks to notice improvement For gut health (IBS, leaky gut, ulcers): Dose: 200-400mcg per day Frequency: Once daily, preferably morning on empty stomach Duration: 6-12 weeks Injection site: Systemic (abdomen) Results timeline: 3-6 weeks For general wellness and prevention: Dose: 150-300mcg per day Frequency: Once daily Duration: Ongoing or 4-week cycles with 2-week breaks Injection site: Systemic (abdomen) Results timeline: Subtle, cumulative benefits Body weight-based dosing: 150 lbs (68kg): Low: 204mcg (3 mcg/kg) Medium: 340mcg (5 mcg/kg) High: 476mcg (7 mcg/kg) 180 lbs (82kg): Low: 246mcg (3 mcg/kg) Medium: 410mcg (5 mcg/kg) High: 574mcg (7 mcg/kg) 200 lbs (91kg): Low: 273mcg (3 mcg/kg) Medium: 455mcg (5 mcg/kg) High: 637mcg (7 mcg/kg) Start at the low end for your goal