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We found that intracellular 1-MNA and NNMT protein expression levels were substantially higher in the fully differentiated adipocytes compared to the undifferentiated pre-adipocytes, which is consistent with the differentiation-dependent enhancement in NNMT mRNA levels and enzymatic activity reported previously in the 3T3-L1 cell lines.[37] The higher levels of 1-MNA and NNMT in adipocytes compared to pre-adipocytes may partially account for our observations that increasing concentrations (10 60 M) of NNMT inhibitor did not completely eliminate 1-MNA in adipocytes, but did fully suppress 1-MNA accumulation in undifferentiated pre-adipocytes

A marked inter-patient variability in purinergic metabolites emerged as a consistent feature of the cohort

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The term alkylene as used herein refers to straight and branched chain alkyl linking groups, i.e., an alkyl group that links one group to another group in a molecule

An increased level of NNMT can lead to various negative effects and illnesses