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N Engl J Med 361:16511661

Local resolution was estimated for each reconstruction using the RELION implementation of local resolution algorithm 35 , and locally scaled maps were used for model building and refinement in all cases

While you freeze the powder, you should never freeze the reconstituted liquid

Andreeff M, Borthakur G , Zeng Z, Kelly MA, R-Y W, McQueen TJ, Qiu Y, Mak D, Burger JA, Daver NG, Pemmaraju N, Kadia TM, Jabbour E, Pierce S, Ravandi F, Garcia-Manero G, O'Brien SM, Cortes JE, Kantarjian HM, Konopleva M

Diodati JG, Dakak N, Gilligan DM, Quyyumi AA

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