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We conducted an a priori power analysis using G*Power Software for independent samples Students t-test 22 , assuming the group difference in mean change of the score is 2 (standard deviation 1.3) 7 and aiming to detect a significance level of 1% and statistical power of 90%, a total sample size of 30 participants (15 in each group) was needed

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Nicotine has known pathological effects, mainly related to the excessive release of catecholamines, endothelial dysregulation and increased insulin resistance.90 Nicotine in electronic cigarettes has been shown to increase heart rate after overnight abstinence.91 Regarding lipid metabolism, nicotine in electronic cigarettes has been shown to increase the amount of circulating saturated fatty acids, decreasing the content of unsaturated fatty acids and inducing insulin resistance

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