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Briefly, frozen-thawed spermatozoa from each group (repeated in triplicated) were collected, centrifuged at 500 g for 10 min, and diluted with phosphate buffer saline (PBS) solution

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Although liver biopsy provides a definitive diagnosis, it is generally not recommended and should be approached with caution due to the risk of bleeding

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2d) confirmed that normalized to general protein levels, amounts of all core histones were indeed reduced by irradiation in a dose-dependent manner, with histones overall showing, on average, a reduction of 22% with 6 mGy/h and 41% at 20 mGy/h chronic radiation for 7 days (Fig