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The immune complexes were incubated with protein A/G magnetic beads for 2 h at room temperature followed by washing with PBST (PBS + 0.05% Tween 20) to remove the unbound immune complexes

Additionally, we tested for interactions between sex and each deficiency as exploratory analyses

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Notably, brain accumulation of these compounds was significantly increased in ischemic models, attributable to compromised blood-brain barrier integrity

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