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Fastp software (v0.20.0) was used to trim the adapter and remove low-quality reads to get high-quality clean reads

Acknowledgements The work of the author has been supported in part by grants AI072648 and AI122176 from the National Institutes of Health, the Phillips Lupus and Autoimmunity Centre of Excellence, and the Central New York Community Foundation

2-D electrophoresis was performed to elucidate the reasons for this discrepancy, and the results indicated obvious changes in central metabolism and amino acid metabolism in the penta-mutant

Nevertheless, further research is required to validate this hypothesis

This dysbiosis may indirectly promote ferroptosis by impairing microbial-derived antioxidant defenses and enhancing oxidative stress, contributing to IBD progression (197)

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