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Western Blot Analysis Cellular and cerebral samples were lysed in RIPA (Beyotime, China) containing inhibitor cocktail (Roche, Germany) for 20 min, following which the lysates were centrifuged (12,000 g) at 4C for 15 min

Ferris RL, et al

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fixed modification, Carbamidomethylation

This confirms other studies in which it was shown that patients with inactive GHRH receptor, due to mutations, still had rhythmic GH secretion, suggesting that another factor, in addition to GHRH, was acting on pituitary GH secretion (79)