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The ischemic damage to the ETC increased both the capacity and the net production of H 2 O 2 from complexes I and III and sets the stage for an increase in ROS production during reperfusion as a mechanism of cardiac injury [47]

In the first case, the study of the subjects who had suffered starvation in the womb, documented that at the age of 60, they had alterations in the methylation of the gene coding for IGF-2 and the entire genome [60,61]

In some experiments, glutathionylated proteins were detected by probing membranes with streptavidin-HRP diluted 1:25,000 in 1% BSA

Prez, R

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