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1a,b and Extended Data Table 2) 35,36

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In the study of Tagitinin C-induced ferroptosis in colorectal cancer cells, it was found that the mechanism of action of Tagitinin C might be related to the activation of the PERK-Nrf2-HO-1 signaling pathway induced by ER stress, leading to ferroptosis (84)

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After washes in PBS, cells were stained for 30 min at room temperature with a filtered Oil Red-O (Sigma) solution (0.5% Oil Red-O in isopropyl alcohol), washed again in PBS, and visualized under an inverted microscope (Olympus)