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How fast B12 deficiency can set in, the earliest signs to watch out for, why standard blood tests can miss it, and just how dangerous it can be left untreated

To make excitatory neurons or astrocytes sensitive to blue light, 1 h after plating (DIV 0 and DIV 2 for neurons and astrocytes, respectively) Ngn2 -hiPSCs or astrocytes were transduced with an adeno-associated virus (AAV) serotype 2 encoding Channelrhodopsin-2 (ChR2) (AAV2-hSyn-hChR2(H134R)-mCherry, UNC Vector Core) 74 and AAV serotype 2 encoding ChR2 (AAV-GFAP-hChR2(H134R)-mCherry, UNC Vector Core) 44,75 , respectively