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After several washes with distilled water, bands corresponding to PDHc-E2 (~75 kDa) or OGDHc-E2 (~55 kDa) were excised using a sterile scalpel and transferred to Protein LoBind tubes (Eppendorf)

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Too much vitamin A can cause: abnormal bone growth, particularly in the spine and joints which can cause stiffness, pain and limited mobility liver damage dry and rough skin which can lead to poor coat condition neurological symptoms such as lethargy, irritability and even seizures vomiting constipation weight loss Too much vitamin D can cause: hardening of tissues and organs, including the kidneys, heart and blood vessels organ failure death Can I use human vitamins for cats

10.1002/ffj.3605 38 ChenY.ChengS.DaiJ.WangL.XuY.PengX.et al

Plus, since vitamin B-12 levels arent routinely checked when you get your blood tested at check-ups, you may not know that youre deficient until you begin experiencing more extreme symptoms