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Crossref Ibrahim SO, Ayipo YO, Lukman HY, et al

Adipose tissuederived cytokines and the increased oxidative burden associated with higher BMI may therefore exacerbate the inflammatory phenotype observed in PCOS rather than act as primary causal factors

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PLoS One 2011

Immunoblotting Proteins were extracted from cultured SCD erythroblasts or spleen Ter119+ erythroblasts with modified RIPA buffer (150 mM NaCl, 50 mM Tris, pH 7.5, 1% NP40, 0.25% Na-deoxycholate) plus protease inhibitor cocktail (Sigma) and stored at 80 C up to processing