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10.1111/jcmm.16693 55 WangL.WangN.ZhangW.ChengX.YanZ.ShaoG.et al (2022)

The precipitated protein was rinsed with 1 ml ice-cold acetone with 0.1% HCl, and centrifuged at 8,500 g for 5 min at 4C

Post-ischemic upregulation of C1q and C3 was also observed by other researchers in models of transient MCAO in adult animals [4, 33, 34] and after experimental neonatal hypoxiaischemia [10, 35,36,37,38,39]

In contrast, the limited diversity observed in most Mediterranean populations may reflect the relatively recent invasion of the species in the basin, which likely involved a limited number of founding individuals