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Taken together this data suggests that hexose phosphates, and not glycerol, are essential for multi-cycle replication ex vivo and full virulence during in vivo infection

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Biofilms were incubated with the stains for 30 min in the dark at RT, then gently washed twice for 5 min each with 2 mL PBS by careful pipetting down the sides of the wells

Randomised, controlled trial of N-acetylcysteine for treatment of acute exacerbations of chronic obstructive pulmonary disease [ISRCTN21676344]

The solution then goes through several stages of filtering, centrifuging, and extracting using solvents like acetone, methanol, ethanol, isoproponal and other chemical products (Pasquel, Meireles, Marques, &Ptenate, 2000)