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Perhaps most importantly, this patent also broadly defined inteins as CIVPSs and identified them based on specific conserved features (Figure 2), thereby providing prior art that would apply to new intein sequences that had not yet been discovered and many of their applications ( FIGURE 2 The methods described by this patent had revolutionary implications in protein engineering

Diabetes Metab Res Rev (2000) 16(4):2306

discovered that giving rats 250 mg/kg of valproic acid, decreased the size of myocardial infarcts by 50 % and raised systolic blood pressure by blocking histone deacetylase and increasing the expression of the Foxm1 gene, which led to more cardiomyocyte cells living (Tian et al., 2019)

Identification of a new tamoxifen-xanthene hybrid as pro-apoptotic anticancer agent

Meng F, Wang K, Aoyama T, Grivennikov SI, Paik Y, Scholten D, et al

Birkhauser Verlag, Basel Klaassen CD, Liu J, Chaudhari S (1999) Metallothionein: an intracellular protein to protect against cadmium toxicity