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The digested tissues were washed three times in PBS, collected by centrifugation (800 g for 10 min), placed into tissue culture dishes (Falcon Labware, NJ, USA) and resuspended in high-glucose DMEM, supplemented with 10% FBS and 50 g/ml gentamycin, and maintained at 37 C with 5% CO 2

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Warm regards, Dr